Immunohistochemistry Resource Guide
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Table of Contents
Immunohistochemistry Selection Guide
Avidin-Biotin Complex (ABC)-Based Detection
Choosing a VECTASTAIN® ABC Kit
> Customizing your VECTASTAIN® ABC Kit
ImmPRESS® One-Step Polymer Systems
> ImmPRESS® Two-Step Amplified Polymer Systems
> Choosing an ImmPRESS® Polymer Kit
> Multiple Antigen Labeling Simplified
M.O.M.® (Mouse on Mouse) Immunodetection Kits
> Counterstain/Substrate Compatibility Table
Enzyme Substrate Properties
See also Enzyme Substrate Combinations for multiple antigen labeling and Counterstain/Substrate Compatibility.
Substrate | Color | Catalog Number | Microscopy | Mounting | Contrast in Pigmented Tissue | Multiple Labeling | Heat Resistant* | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|
Bright- field | Darkfield | Electron | Fluore- scence | Spectral Imaging | |||||||
Peroxidase | |||||||||||
Vector® DAB | Brown | • | • | • |
| • | Non-aqueous or Aqueous |
| • | • | |
Vector® DAB +Ni | Gray-Black | • | • | • |
| • | Non-aqueous |
| • |
| |
ImmPACT® DAB | Brown | • | • | • |
| • | Non-aqueous or Aqueous |
| • | • | |
ImmPACT® DAB EqV | Brown | • | • | • |
| • | Non-aqueous or Aqueous |
| • | • | |
Vector® VIP | Purple | • | • | • |
| • | Non-aqueous | • | • |
| |
ImmPACT® VIP | Purple | • | • | • |
| • | Non-aqueous | • | • |
| |
Vector® SG | Blue-Gray | • | • | • |
| • | Non-aqueous or Aqueous | • | • |
| |
ImmPACT® SG | Blue-Gray | • | • | • |
| • | Non-aqueous or Aqueous | • | • |
| |
Vector® NovaRED® | Red | • | • | • |
| • | Non-aqueous | • | • |
| |
ImmPACT® NovaRED® | Red | • | • | • |
| • | Non-aqueous | • | • |
| |
Vector® AEC | Red | • |
|
|
| • | Aqueous | • | • |
| |
ImmPACT® AEC | Red | • |
|
|
| • | Aqueous | • | • |
| |
ImmPACT® AMEC Red | Red | • |
|
|
| • | Aqueous | • | • |
| |
TMB | Blue | • |
|
|
| • | Non-aqueous |
|
|
| |
Alkaline Phosphatase | |||||||||||
Vector® Red | Magenta | • |
|
| • | • | Non-aqueous or Aqueous | • | • | • | |
ImmPACT® Vector® Red | Magenta | • |
|
| • | • | Non-aqueous or Aqueous | • | • | • | |
Vector® Blue | Blue | • |
|
| • | • | Non-aqueous or Aqueous | • | • | • | |
Vector® Black | Brown-Black | • |
|
|
|
| Non-aqueous |
|
|
| |
BCIP/NBT | Indigo | • |
|
| • | • | Non-aqueous or Aqueous |
| • | • | |
* Substrates that are designated “heat resistant” were developed on tissue then subjected to heat induced epitope retrival (HIER) using a pressure cooker technique (stained tissue was pressure cooked for 1 minute in Antigen Unmasking Solution, returned to room temperature, and rinsed in buffer). Resulting sensitivity after this treatment was found to be equivalent to non-HIER treated tissue.
Prostate: Prostate Specific Antigen (m), ImmPRESS Reagent (HRP), ImmPACT DAB (brown).d), Hematoxylin QS (blue).
Tonsil: Cytokeratin AE1/AE3 (m), ImmPRESS Reagent (HRP), Vector DAB-Ni (gray-black).
Tonsil: Cytokeratin AE1/AE3 (m), Vector ImmPRESS- AP Reagent, ImmPACT Vector Red (magenta).
Tonsil: Cytokeratin AE1/AE3 (m), ImmPRESS Reagent (HRP), ImmPACT SG (blue-gray).ylin QS (blue).
Tumor: Cytokeratin (s), VECTASTAIN® Elite® ABC Kit, TMB (blue).
Tonsil: Cytokeratin AE1/AE3 (m), ImmPRESS-AP Reagent, Vector Blue (blue).
Tonsil: Cytokeratin AE1/AE3 (m), ImmPRESS Reagent (HRP), ImmPACT NovaRED (red).EC (red), Hematoxylin QS (blue).
Prostate: Prostate Specific Antigen (m), ImmPRESS Reagent (HRP), ImmPACT AEC (red).
Colon Carcinoma: Pan-Cytokeratin (m), VECTASTAIN ABC-AP Kit, Vector Black (brown-black).
Tonsil: CD20 (m), ImmPRESS Reagent (HRP), ImmPACT VIP (purple).
Tonsil: LCA (m), ImmPRESS Reagent (HRP), ImmPACT AMEC Red (red).
Prostate: Prostate Specific Antigen (m), VECTASTAIN ABC-AP Kit, BCIP/NBT (indigo).
